Which method is primarily used for gene editing?

The primary method used for gene editing among the listed options is CRISPR technology. CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, is a revolutionary tool that allows scientists to precisely alter DNA within living organisms. It works by using a guide RNA to direct the Cas9 enzyme to a specific location in the genome where it can make cuts, allowing genes to be knocked out, added, or modified with high efficiency and accuracy.

This technology enables advancements in various fields such as medicine, agriculture, and biology by providing a straightforward and adaptable way to edit genetic material. Its ability to target specific sequences enhances both research applications and therapeutic approaches for genetic diseases.

In contrast, the other methods mentioned serve different purposes. Polymerase chain reaction (PCR) is used for amplifying DNA but does not edit genes. DNA profiling is primarily used for identification purposes, such as in forensics and paternity tests, rather than modifying the genetic material itself. Genetic cloning focuses on creating identical copies of an organism or DNA segment and, while related to genetics, does not involve direct editing of the gene sequences like CRISPR does.